AFM University Introduction to Atomic Force Microscopy by Paul West

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6.1 Probe Artifacts
6.1.1 Features on a Surface Appear Too Large
6.1.2 Features in an Image Appear Too Small
6.1.3 Strangely Shaped Objects
6.1.4 Repeating Strange Patterns in an Image
6.2 Scanner Artifacts
6.2.1 Probe / Sample Angle
6.2.2 X-Y Calibration / Linearity
6.2.3 Z Calibration / Linearity
6.2.4 Background Bow / Tilt
6.2.5 Z Edge Overshoot
6.2.6 Scanner Drift
6.2.7 X-Y Angle Measurements
6.2.8 Z Angle Measurements
6.3 Image Processing
6.3.1 Leveling
6.3.2 Low Pass Filter
6.3.3 Matrix Filter / Smoothing
6.3.4 Fourier Filtering
6.3.5 Image Looks Too Good
6.4 Vibrations
6.4.1 Floor Vibrations
6.4.2 Acoustic Vibrations
6.5 Other Sources
6.5.1 Surface Contamination
6.5.2 Electronics
6.5.3 Vacuum Leaks
6.5.4 PID Settings / Scan Rate
6.5.5 Laser Interference Patterns
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FIGURE 6-22 Low pass filtering of the step on the left (A) results in the shape shown on the right (B). The amount of distortion depends on the amount of filtering applied to the image.
When images are viewed that have substantial low pass filtering, the dimensions in the image can appear distorted. Other artifacts can appear as a sharpness at the edge of steps in an image.
6.3.3 Matrix Filter / Smoothing
Matrix filtering is very effective at "smoothing" images and removing noise from the image. However, the filtering process often reduces the resolution of the image. As a rule of thumb, if the image has no noise in it, then the data has probably been compromised.
6.3.4 Fourier Filtering
Periodic structures can easily be introduced into images with Fourier filtering. This can be used for creating "atomic structure" in images. As an example, images of "white noise" can be filtered to give periodic structure that looks like atomic structure.
FIGURE 6-23 A: AFM image of nanospheres with no filtering. The image shows noise in the associated line profile. B: The image generated after matrix smoothing. The line profile shows no noticeable noise and the shape of the particle is altered.

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